Increasing the accuracy of my program

Anyone here who can help me with the calibration of this program: https://www.dropbox.com/s/oq6nx1r9cg0ikh3/Nitrogen%20Def.rar

All the images that I used are already there. My program is already working but I'm not getting accurate results. The 10 leaf images included is supposed to be Panel 4 but when I run it on my program, it gives me an output of sometimes Panel 2, sometimes Panel 3. I've already tried calibrating the mask for both the LCC and the Test images but still the same result.

Thanks