I have a housekeeping gene [2 4 6 8 10]
How do i work out the difference between each measurement of the housekeeping gene and the mean of housekeeping gene?

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I think you're asking how to take the values [4 8 6 104 5] and subtract the mean?
a = [4 8 6 104 5];
b = a-mean(a)
b = 1×5
-21.4000 -17.4000 -19.4000 78.6000 -20.4000

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