This is the snippet for ROI I used threshold intensity for background as less than 4 and i get ROI but histogram for both original and roi image is same. so what's wrong here ? I used window of 3by3 or 5by5 to get roi

      clc;
      close all;

      a= imread('20051019_38557_0100_PP(1).tif');
      [m n]=size(a); 

      N=input('enter the window size=');

      %The window size can be 3x3,5x5etc Start=(N+1)/2; 
      Out_Imag=a; 
      for 
         i=Start:m-Start+1, 
           for 
             j=Start:n-Start+1, 
               limit=(N-1)/2;
               Sum=0;
                 for k=-limit:limit,
                    for l=-limit:limit,
                       if(a(i+k,j+l)<=4)
                       %Out_Imag(i,j)=255;
                       Out_Imag(Out_Imag(i,j) == 255) = NaN;
                       %else 
                         % Out_Imag(i,j)=255;
                      end 
                   end 
               end 
            end
       end

           original=rgb2gray(a);
           imshow(original),title('original Image'),figure, 
           imhist(original),title('original hist Image'),figure, 
           %Out_Imag(Out_Imag(i,j) == 255) = NaN;
           roi=rgb2gray(Out_Imag); imshow(roi),title('roi Image'),figure, 
           imhist(roi),title('roi_hist Image')
           % imshow(uint8(a)),title('original Image'),figure, 
           % imshow(uint8(Out_Imag)),title('ROI Image');
           % InHistgray=rgb2gray(a); 
           % imhist(histgray),title('Histogram of original Image'),figure, 
           % OuyHistgray=rgb2gray(a); 
           % imhist(OuyHistgray),title('Histogram of ROI Image');

[Image Analyst]

I can't read it. See http://www.mathworks.com/matlabcentral/answers/13205-tutorial-how-to-format-your-question-with-markup

Answer 1

[Shriram Nerkar]

Please find attachment which contains image file and matlab script run the script.

• Item one after results in last image some part of region of interest is removed. help me to sort out
• Item two any suggestion criticism highly appreciated.