How to track an object from a 3D tiff images?

Dear all,
I have tiff image sequences generated from a confocal microscope of X,Y,Z. I would like to track one object (nucleus) that moves over the time in the X,Y,Z direction. Does anyone knows how to track such a moving object from those tiff images (8 bit gray scale)?
Any suggestion is welcome.
Best, Meshoo

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Question posée :

le 26 Mai 2015

Réponse apportée :

le 27 Mai 2015

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