Undefined function or variable 'im'.

Also I got error for " imM=im>80;" in pushbutton2 function

Code:

% --- Executes on button press in pushbutton1.

function pushbutton1_Callback(hObject, eventdata, handles)

[fname path]=uigetfile('*.jpg');

fname=strcat(path,fname);

im=imread(fname);

im=rgb2gray(im);

axes(handles.axes1)

imshow(im);

title('Grayscale Iris Image');

% --- Executes on button press in pushbutton2. function pushbutton2_Callback(hObject, eventdata,handles)

imM=im>80;

axes(handles.axes2)

imshow(imM);

title('Threshold Image');

Answer 1

[Image Analyst]

I answered this already in your other question. Basically, see the FAQ http://matlab.wikia.com/wiki/FAQ#How_can_I_share_data_between_callback_functions_in_my_GUI.28s.29.3F to see a variety of ways how you can get "im" into that function.

[Hazel Sialongo]

I got error again says, " imM=im>80;" in pushbutton2 function. Can you please help me?

[Hazel Sialongo]

By the way, thank you for helping me solving my first problem.

[Image Analyst]

I guess you didn't want to read the FAQ, or didn't understand it. If you didn't understand it, probably the simplest approach for you is to simply put

global im;

as the first line of every function where you expect to use im. Just be aware that if you change it in any function, it's global so it will change in all functions from that point in time onwards. Functions that have that global line will see im, and functions that don't have that line won't see im.