Extracting white blood cell from cell samples

Hi,

I am working on leukemia images. http://www.clinicalflow.com/Cases/Case_List/Acute_Lymphoblastic_Leukemia_%28ALL%29_general.

I need to extract only the purple cells, which are also the largest cells as opposed to others. Plz let me know how to extract only those cells , and also how i can find the area, mean and intensity of these cells alone. Kindly help..

Thank you,

Mona

[Rumana Afrose]

Hello,

Do u have classified the White Blood Cells?

Answer 1

[Sarah Wait Zaranek]

This should be enough to get you started. I filtered the purple cells by looking at the blue color plane. I then removed any noise - and used region properties to get the area of the cells. You can use region properties to get a list of pixels and use that to calculate mean values from the original image.

%%Reading in the image
myImage = imread('L1+blast.jpg');
figure
imshow(myImage)

%%Extracting the blue plane 
bPlane = myImage(:,:,3)  - 0.5*(myImage(:,:,1)) - 0.5*(myImage(:,:,2));
figure
imshow(bPlane)

%%Extract out purple cells
figure
BW = bPlane > 29;
imshow(BW)

%%Remove noise 100 pixels or less
BW2 = bwareaopen(BW, 100);
imshow(BW2)

%%Calculate area of regions
cellStats = regionprops(BW2, 'all');
cellAreas = [cellStats(:).Area];

%%Superimpose onto original image
figure, imshow(myImage), hold on
himage = imshow(BW2);
set(himage, 'AlphaData', 0.5);

[Ahmed]

Hai..

than how can we counting the number of cell after image BW=rPlane.

I adjust the program to find the red cell.

%% Reading in the image

clear all

myImage = imread('L1 blast.jpg');

figure

imtool(myImage)

%% Extracting the red plane

rPlane = myImage(:,:,1)- 0.4*(myImage(:,:,3)) - 0.6*(myImage(:,:,2));

figure

imshow(rPlane)

%% Extract out red cells

BW = rPlane > 19;

figure

imshow(BW)

http://www.flickr.com/photos/67515191@N07/6149005257/

http://www.flickr.com/photos/67515191@N07/6149555552/

[sana saleeme]

and what should we do if we want to extract the red blood cells?

[murk hassan memon]

i am facing this type of error when i am converting gray from original and then extracting blue pan kindly help me to resolve this problem and if i am making any mistake then also tell me the write way to code

[Navin Kumar]

Hi,how can i segment cells and nucleus separately?By applying the above code,only the nucleus get segmented.So i applied lab color space and kmeans.For some images only i got correct answer,please let me know how to extract cells.