Regarding the watershed transform.

This is my input Image Input Image

I want to seperate the individual chromosomes from this image. I have used watershed transformation. I got the output as Output Image.

Is there any possibilty to seperate the regions of chromosomes from here???

My code is

inputImage = imread('chromosome.jpg');
figure, imshow(inputImage);
bw = im2bw(inputImage,0.8);
figure, imshow(bw), title('bw')

D = bwdist(~bw);
figure, imshow(D);
title('Distance transform of ~bw')

D = -D;
D(~bw) = -Inf;

L = watershed(D);
rgb = label2rgb(L,'spring');
figure, imshow(rgb);
title('Watershed transform of D');

Answer 1

[Image Analyst]

What does that mean? Watershed does separate them and give you a labeled image. So what's the problem? http://www.mathworks.com/matlabcentral/answers/7924-where-can-i-upload-images-and-files-for-use-on-matlab-answers

[Sabarinathan Vadivelu]

My problem is to seperate the individual chromosome images in there. The process is called chromosome karyotyping

[Image Analyst]

If watershed is not doing the job for you then you need to use an algorithm that people have used successfully and published, like those in section 20.4 here: VisionBib