after doing arithmatic operations to the image is there any method to convert the result into the image.

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Image is nothing but a matrix of data. You can write any matrix into an image by using
imwrite
method.

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but some times arithmatic operations gives negative values.is it a problem for displaying the image.
Cast to double before subtracting, then use imshow(yourImage, []) to display all the image, both positive and negative pixels.

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