Remove object from plot

Hello People, i have following code

pic01=imread("1.tiff");
pic1=im2gray(pic01);
i=1;
boundaries=[];
for i=3:25:222;
pic050=im2gray( imread(i+".tiff") );
%pic50=im2gray(pic050);

newpicture=imsubtract(pic01,pic050);
%imshow(newpicture)
level = graythresh(newpicture);
level = 0.19;
newbinpic = imbinarize(newpicture,level);
%imshowpair(newpicture,newbinpic,'montage')
newbinpic2=bwpropfilt(newbinpic,'perimeter',1);
% imshowpair(newbinpic2,newpicture,'montage'); 
boundariesi = bwboundaries(newbinpic2,'noholes');
boundaries=[boundaries;boundariesi];
binaryImage = 0.6< newbinpic2 & newbinpic2<1;



end

%figure; 
%imshow(pic01);
t = 0:1:2000; %setup time
clors= hsv(numel(t)); %create a color map of different color for each time
figure(1),clf,hold on
%hold on
t1=1;

for k=1:length(boundaries)
thisBoundary = boundaries{k};
x = thisBoundary(:,2);
y = thisBoundary(:,1);
plot(x,y,'color',clors(t1,:),'Linewidth',2);
t1 = t1+10;
end
axis off
grid off

Which takes a series of photos and creates this 'plot' out of it

My question is, does anyone maybe have an idea on how to remove the nozzle (this thing in middle) along with the tube it kinda ruins the plot i think. Thanks very much bois and girls!

Answer 1

[Image Analyst]

Attach 3 or 4 of the images with paperclip icon. We ca't run your code without it. I have done this before where a tube is dripping a colored liquid onto a surface and we need to erase the tube. It's bit tricky especially if the stain grows out and then circles back around under the tube. You need an image with the tube (filled with liquid if it's transparent) but not with any stain on the surface yet. It mostly involves logical and morphological operations like convex hull, AND, OR, imfill(), etc.

[Alex Perrakis]

Hello, thanks for your answer, without probably knowing it you have helped me alot during this project (except the fact that you already answered another question of mine). I have a lot of photos that i used for that plot so maybe you need more , so i uploaded 6. Thanks again

P.S. My original photos are in .tiff but mathworks does not accept this format so i upload in png.

[Image Analyst]

Here is a start (see attached). I used the 22 image after I saved it and changed the extension to tif.

[Alex Perrakis]

Thank very much! You helped me a lot! May i ask you one more question? I tried to make that code you sent me into a loop and until one point in functioned really well, but the masked "stain" goes black for some reason, is this because of the threshold?

[Image Analyst]

Could be. Just check the masks every step of the way by calling imshow() and see where and why it goes all black.

Another thing you should do is a background correction because you have quite severe nonuniformities due to lighting or lens shading. You need to estimate the background and divide the test images by the background. See attached demo.