How can i set same intensity of two different RGB image?

I want the white blood cell.when i set threshold for one,other does not work properly.can i find a code to set one thershold/intensity for grtting the WBC?

Answer 1

[yanqi liu]

may be use hsv or lab colorspace to segment，such as

im = imread('https://ww2.mathworks.cn/matlabcentral/answers/uploaded_files/917019/8.jpg');
jm = rgb2hsv(im);
s = mat2gray(jm(:,:,2));
s = imcomplement(s);
h = mat2gray(jm(:,:,1));
bw = imbinarize(s,'adaptive');
bw = bwareafilt(imfill(imclose(bw, strel('disk', 10)), 'holes'),1);
bw2 = imbinarize(h,'adaptive','ForegroundPolarity','dark','Sensitivity',0.9);
bw2(bw) = 0;
figure; imshow(im);
hold on; h = imshow(label2rgb(bwlabel(bw)), []);
set(h, 'AlphaData', 0.5)

Answer 2

[Image Analyst]

Use the Color Thresholder app on the Apps tab of the tool ribbon. Use HSV color space and then Export the function, and then you can call it from your main program.