How to plot line profile for a image

I have a image like this and wanted to make a line profile for this image

This image is a maximum intensity projection of a 3d image in XZ direction

Answer 1

[Image Analyst]

Uh, okay, but what's the question? Is that an RGB image, or an indexed (pseudocolored) image?

To get an average vertical profile you can do

verticalProfile = mean(grayImage, 2);
horizontalProfile = mean(grayImage, 1);

To get a profile along any specific row or column you can do this

verticalProfile = grayImage(:, columnNumber);
horizontalProfile = grayImage(rowNumber, :);

To get the profile between any two points you can use improfile.