Scale Bar adjustment in an OCT scan

Greetings,

Can somebody please help me with an Issue?I am trying to measure the area of lined ROI using FIJI since I am quite noobie in MATLAB, however as you can see the width and height of the scale bar are not similar, and I don’t know how I can change the pixel measurements to microns since the set scale option only has one bar to fill.

Is there anyway I could fix the problem without losing the scan details?

Scale Height = 51.34 Px

Scale Width = 16.83 Px

Answer 1

[Image Analyst]

Perhaps you should ask your Fiji question in an ImageJ forum. We're just about MATLAB here. I'm attaching a demo about drawing a polygon in MATLAB.