Superimposing Plotted Data on the image

Dear All

I am trying to plot curve data of X and Y coordinates on the image using plot and imagesc. However, i can see the plotted data on the image with different scaling and reference start point.

Let say, my ROI(Region of Interest) is 188 x 210 pixels and the no of points in X and Y are 103 in each in terms of pixels. But the final figure shown in here http://imageshack.us/photo/my-images/822/finalcurvefittingforult.jpg/ is out of the original image edge. I think it will be because of scaling with plot and imagesc as well some reference starting points.

Pls advise me since I was stuck on it and looking forward to hearing from you good news soon.

Thanks and best regards Kyaw