Calculate time intensity curve of ROI (multiple images)

Hi everyone,

I'm analysing a video (I splitted the video into grayscale frames) and I would like to perform the following things: 1. Determine three ROIs by hand, save the ROIs and use them for all images. 2. Calculate the mean value of the ROIs in each image 3. Create a time-intensity graph of al images

    % code
  im = VideoFrames_Fluo(:,:,k);
        imshow (im);
        ROI = imellipse();
        BinaryImage = ROI.createMask();
        numberOfPixels1 = bwarea(BinaryImage(:));% Calculate area
        structBoundaries = bwboundaries(BinaryImage);
        xy = structBoundaries{1};
        x = xy(:,2);
        y = xy(:,1);
        m = mean2(BinaryImage);

I also found the mmROI code, however I cannot get to work. Could someone help me please?