I are working on a project 'WBC and RBC detection and counting'. I need to count the RBCs alone from the blood smear image after removing the WBCs. I need a method to remove those WBCs from the image.

My project work deals with counting of RBCs and WBCs. I got succeeded in counting the WBC by extracting the WBC nucleus. I tried counting RBCs by using Hough transform-circlular shape detection. But the problem is that along with RBCs ,WBCs are also getting detected. So I tried removing the WBCs from the image and then to count the RBCs alone. I did remove the WBC nucleus from the image. But the same problem exists again. The WBCs are again detected because I am not able to remove the cytoplasm around the WBC nucleus.Hence the cytoplasm also get detected as circles. So I need a help to remove the WBC cytoplasm along with the nucleus so as to detect the RBCs alone. Here is the program that I have done:

clc;
clear all;
close all;
rgb = imread('smear7.jpg');
figure;
imshow(rgb);
gray_image = rgb2gray(rgb);
figure;
imshow(gray_image);
[size1,size2]=size(gray_image);
for i=1:size1
   for j=1:size2
      if gray_image(i,j)<100
          gray_image(i,j)=255;
      end
   end
end
%%Nucleus removal

im1=im2bw(gray_image,graythresh(gray_image));
figure;
imshow(im1);
figure;
imshow(im1);
[centers, radii, metric] = imfindcircles(rgb,[5 10],'ObjectPolarity','dark','Sensitivity',0.95,'Method','twostage');
h = viscircles(centers,radii);
[m,n]=size(centers);
disp(m); %RBC COUNT

The image attached here is one among the blood smear images that I am using.

[SERIN JOSE]

Thank you sir

[Suraj Gore]

I want wbc count matlab code

Answer 1

[Image Analyst]

I'd use color segmentation instead of imfindcircles(). See my tutorials: http://www.mathworks.com/matlabcentral/fileexchange/?term=authorid%3A31862

Answer 2

[Marikit Avendaño]

Hi! I wanted to ask if you have successfully counted the RBC and removed the WBC completely?

[Image Analyst]

Hard to tell since he said "Thank you" but never accepted my answer.

But I'd bet it's solved since he never wrote me back with any follow up questions.

Answer 3

[murk hassan memon]

Hi SERIN JOSE . i have tried your code but here is my question to u? In general as we all know and i have also studied that red blood cells become shorter after bitten by an infected mosquito (malaria infection) and on the comparison basis Normal/ uninfected red blood cells are higher than Abnormal/infected red blood cells. so when i have implemented your code on both Normal and Abnormal blood cells the results not quite seems a valid like in some Abnormal blood images the majority red blood cells are very high than Normal red blood cells. so this is actually my confusion i want to clear my concept regarding this whether infected red blood cells become lower or not? your suggestion/answer will be appreciated thanks