Could you please help me related to identify exactly central coordination of bright Objects in an image. For example, in image below, i want to know the exact position of each LED. Thanks for your help.

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Image Analyst
Image Analyst le 29 Août 2017
Modifié(e) : Image Analyst le 29 Août 2017

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Try this:
binaryImage = grayImage > 128;
[labeledImage, numSpots] = bwlabel(binaryImage);
props = regionrops(labeledImage, 'Centroid');
props is a structure array where Centroid is a field of the structure. You can get a table variable out instead of a structure array if you want.

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