Effacer les filtres
Effacer les filtres

segmentation of some overlapped portions in the cell image ?

2 vues (au cours des 30 derniers jours)
Sumit Chaudhary
Sumit Chaudhary le 10 Mai 2018
In the attached output image, there are some red boundaries which contains more than one blue asterik, here i need to segment the overlapped portion such that each red boundary contain one blue asterik. If there are two blue asterik in a red boundary, then segement that red boundary into two boundaries such that each boundary has blue one asterik and simlairly if more than two blue asterik are present in a red boundary.
I have thought of a technique but unable to implement it in matlab. Lets take the case of two blue asterik in a red boundary. I will join the two blue asterik by a straight line and then draw a line perpendicular(lets name it as line 1) to it. Now, find the point of intersection of the line 1 and red boundary. draw two line parallel to the line 1 passing the red boundary three points behind and after the point of intersection. Now we will have two closed curve each having one blue asterik. I am not so good at programming, please help me out, Feel free to ask for any clarifications regarding the question.
you have to download the excel file containg the co-ordinate of the blue asterik and the image where i have drawn red boundary, this part i have completed.
clc;
clear all;
close all;
Icell = imread('10_GFP.jpg');
Icellgray = rgb2gray(Icell);
level = 0.18;
Ithresh_cell_1 = im2bw(Icellgray, level);
Ifilled = imfill(Ithresh_cell_1, 'holes');
Ithresh_cell_1 = bwareaopen(Ifilled, 245);
boundaries = bwboundaries(Ithresh_cell_1);
numberOfBoundaries = size(boundaries, 1);
centroid = xlsread('Centriod_nucleus.xlsx')
imshow(Ithresh_cell_1);
hold on;
plot(centroid(:,1), centroid(:,2), 'b*');
hold on;
for k = 1 : numberOfBoundaries
thisBoundary = boundaries{k};
plot(thisBoundary(:,2), thisBoundary(:,1), 'R', 'LineWidth', 2);
hold on;
end
hold off;

Réponses (0)

Community Treasure Hunt

Find the treasures in MATLAB Central and discover how the community can help you!

Start Hunting!

Translated by