App Designer errors if the axes is produced through the App Designer GUI. However, if the axes is produced programatically, App Designer finds the axes correctly. To produce the axes programatically, for example:
app.FullImageUIAxes = axes(app.UIFigure, 'Units', 'Pixels', 'Position',[166,84,750,744], 'XTick', [], 'YTick', []);
Then you can use drawrectangle:
app.ROI = drawrectangle(app.FullImageUIAxes, 'Position', [app.ROIposition(1),app.ROIposition(2),app.ROIposition(3),app.ROIposition(4)], 'LineWidth', [1], 'Color', [1 0 0]);
You have to add listeners programatically since you cannot add them through App Designer in this case:
addlistener(app.ROI,'MovingROI', @(varargin)ROIMoving(app, app.ROI));