How can I get total intensity of particles in noise image?
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Jaehwi Bong
le 5 Mai 2019
Commenté : Image Analyst
le 12 Mai 2019
I have a spinning disk confocal image which has a lot of noise and particle(my interests have differnt size and move slowly over time). I need only intensity information of selected positions over time in diffraction-limited images.
I'm using Track mate to detect particle and get their coordinates.
I want to apply 2D Gaussian fitting to particles which have a different offset of noise. Then, I would measure the intensity of each particle.
What and How can I do for that using Image J or Matlab?
Or could you recommend the best way to analyze intensity fluctuation of particles(3D, timelapse)?
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Image Analyst
le 5 Mai 2019
You can get in a loop where you measure the mean and SD. Attached is an example where I get the mean - make adaptations as needed.
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Image Analyst
le 12 Mai 2019
I haven't looked at the video yet but if you have bright blobs that you want to model as Gaussian peaks, then I'd first see if you can threshold the frame and detect the bases of the blobs. Then ask regionprops for the bounding box. Extract out the subimage of the bounding box and model it to a Gaussian using something like fitnlm or fitgmdist(), in the stats toolbox.
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