suggest a program for finding the length of chromosome in an image.

[Walter Roberson]

http://www.mathworks.com/matlabcentral/answers/7924-where-can-i-upload-images-and-files-for-use-on-matlab-answers

Answer 1

[Image Analyst]

I suggest imdistline() or improfile(), unless you can say why they won't work for you.

[manoj m]

imdistline() is an interactive tool.i want automatic detection of line and its length.improfile() gives only the intensity values not the length info of the line.

the image for which length has to be calculated is: http://imageshack.us/photo/my-images/716/62bt.png/

[Image Analyst]

I'm not sure I understand. That's a skeleton. Just sum it to get its length.

[manoj m]

ok sir i understand.for a single line if we sum we would get the length.i also want to know how to calculate the length of a particular line in an image where two lines intersect each other.how could i separate the lines.For example the image is: http://imageshack.us/photo/my-images/838/chromosomeendpointsandj.jpg/ ,i want to calculate the length along AD,BC,CD,AB.

[Image Analyst]

Use the 'branchpoints' option of bwmorph() to split it apart. Then use regionprops() to get the area of each segment.

[manoj m]

1. Sir,for skeletonized image if its of single pixel width,then sum would give the length.But by skeletonization it is not entirely single pixel width.what should be done to convert it to single pixel width. 2. i have found the 'branch points' for the image that i asked before but i don't know to separate it into segments with that branchpoints. help me on that.

[Image Analyst]

Skeletonization does go down to single pixel if you use the 'inf' option.

Just find the branchpoints and subtract the branchpoint image from the original skeletonized image.