How to measure distance between two lines in an image?

I want to measure the distance between the two red coloured lines marked in the image (Lines are supposed to be straight).

I don't know the pixel location of these lines. The distance is supposed to be in micrometers. How can I do it?

Answer 1

[Image Analyst]

How about imdistline() or improfile()?

[Praveen Kumar Kandavel]

But I don't want to manually enter or choose those coordinates, rather I want to develop an algorithm to programitically choose those coordinates and measure the distance between them.

[Image Analyst]

Can you state in words, exactly where you would place the endpoints of the lines?

[Praveen Kumar Kandavel]

the vertical length of the upper part of brown region (above the bisecting line) in the left side and the horizontal length between the edge and center of the S-curve on the right side as mentioned in the image